global proteome analysis (expression)

Samples: cells, tissues, organelles, subcellular fractions, coIP/ affinity isolated protein complex; guideline protein input: Ideally 10-20 microgram in 50 microliter (though lower protein yields may also work). Depending on workflow, prices range from ~220-230 euro / sample

Key publication: Knol, JC, … Guo* & Jimenez*. The pan-cancer proteome atlas, a mass spectrometry-based landscape for discovering tumor biology, biomarkers, and therapeutic targets. Cancer Cell 2025 May 27:S1535-6108(25)00212-0.

Services label free proteomics

Proteomics

General steps in workflow

Proteomics workflow

Quantitative proteomics by shot-gun proteomics (Data Dependent Acquisition, DDA) and Data-Independent Acquisition (DIA) Mass Spectrometry

Quantitative proteomics by shot-gun proteomics

Protein expression sample processing workflows

Suspension Trapping (Strap)
– Single sample
– 96-well plate

Suspension trapping

In-gel digestion

In-gel digestion

In-solution digestion
– Global biofluid proteomics

In-solution digestion
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